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[img]https://cdn3.volusion.com/pahfr.ubfkh/v/vspfiles/photos/DBB-1.gif?1345373371[/img]A substantial proportion of the swine produced in North America currently are
created using artificial insemination. Hence, the capacity to preserve animal reproductive samples, such as semen, oocytes and embryos, for
use in artificial insemination and other reproductive processes is essential.
Without the need of this capability, it is not probable
to transport animal reproductive samples or shop the samples for any length of time therefore,
the utility of the samples is restricted. To guard reproductive samples from bacterial contamination, it has been frequent practice to introduce antibiotics into the
preservation mediums or extender solutions. Although standard
antibiotics can be a low price and powerful way of preserving the samples, there are extended term consequences of the continued widespread use of antibiotics.
More than time, bacteria come to the fore which are resistant
to antibiotics.

The presence of low levels of antibiotics in agricultural livestock
and human meals items is thought of to be one trigger of the increasingly resistant bacteria.

The increased resistance of bacteria to antibiotics poses a danger
to humans and animals as infectious illnesses are becoming a lot more tricky to treat.

Therefore, in particular regions, standard antibiotics are no longer effective.
Additionally, there is consideration being provided by regulatory
bodies to banning the common use of antibiotics in animals raised for food production. Hence, it is desirable to have an extender
that has antimicrobial properties, but is substantially free of charge
of antibiotics. It is also desirable to have a
system for preserving animal reproductive samples that utilizes
an extender that has antibicrobial properties, but is
substantially free of charge of antibiotics.

The technique of this invention mixes a colloidal suspension of silver as a component of a preservation medium for the preservation of animal reproductive samples.
By adding the colloidal suspension of silver to standard semen extenders, for example,
an extender for boar semen which has antimicrobial properties, but substantially
free of antibiotics is made. A colloidal silver
remedy getting a silver concentration of at least about 1×10−9 moles/liter has been discovered effective in preserving
some reproductive materials. It is an object of the present invention to deliver
an extender with antibacterial and antiviral properties
devoid of the use of antibiotics. Additional objects, functions and
benefits of the invention will be apparent from the following detailed description when taken in conjunction with the accompanying drawings.

A colloidal dispersion of nanoparticles of silver may possibly be used to preserve reproductive samples such as boar semen, producing a medium which is antibacterial and antiviral.
The colloidal dispersion of silver may possibly be developed
by means of the strategies detailed beneath, or any other appropriate technique.
In distinct, a colloidal silver resolution is mixed
with an extender concentrate to type an extender composition. The colloidal
silver solution has a silver concentration of at least about
1×10−9 moles/liter. The silver could be present in one or extra types, which includes ionic, elemental, or a mixture thereof.
Additional, the ions may possibly be present in 1 or much more oxidation states.
The silver ions have an affinity to sulfhydryl
groups in enzyme systems, and by means of which they interfere with the transmembraneous power transfer in bacterial
microorganisms.

The extender composition is then mixed with an animal reproductive sample to type a
biological culture medium beneficial for animal reproduction. The animal reproductive sample is usually semen, oocytes,
embryos or other options usable for reproductive purposes.
Preferably, the animal reproductive sample is boar semen. The
concentration and viability of the animal reproductive sample
is determined by known methods in the art.

Primarily based on the concentration and viability of the animal reproductive sample,
an amount of the extender composition is added to
maximize the number of doses of the biological
culture medium helpful for animal reproduction. The quantity of extender composition to be added to the animal reproductive sample is
determined primarily based on standard procedures in the art.
As is properly identified, the chemical composition of animal reproductive
samples will differ from animal to animal, and even between collections from the similar animal.

Excessive levels of chlorine or nitrogen in the sample, for instance boar semen, will kind
compounds with the silver remedy which are detrimental to the effectiveness of the extender, and at instances even spermicidal.
Therefore, the extender composition of the present invention should really not be used exactly where
chlorine concentrations are a lot more than about
700 ppm. Likewise, if the nitrogen levels in the animal reproductive sample are
also high, the extender composition of the present invention does not work.
The biological culture medium of the present
invention is microbiologically stable at refrigerated circumstances (much
less than about 40° F.) for up to one week. 4 bacterial strains were grown overnight in trypticase soy broth (TSB) at room temperature.

The subsequent day they have been transferred to
TSB (diluted 1:one hundred in distilled water) and grown overnight at
space temperature. The stains have been pooled and
inoculated into water from Aldrich Chemical Co. (32,007-2 ACS grade).
Bacterial stains applied were two Pseudomonas fluorescens a Salmonella typhimurium and an Etrobacter agglomerans.
Immediately after plasma-remedy, the samples have been directly plated or diluted in phosphate buffered saline and then plated
on Trypticase soy agar. The plated were incubated at
room temperature for 72 hours.

To test for injured bacteria, .five ml of each and every
sample was added to four.five ml of SB and incubated at space temperature.
In all solutions resulting from the plasma-treatments the bacteria have been totally
killed. Even solutions ready from 200 ml, initial "living-bacterial soup" and 1 ml of option of the ten seconds plasma-treated bacterial
soup exhibited a 99% reduction of the living bacterial content.

The only samples that were good for growth upon enrichment had been the untreated water
and untreated water with 1 ml of 10 seconds treated bacterial soup.
Treatment of the samples, even for five seconds, killed the bacterial inoculum.
No bacteria had been recovered either by direct plate count or by enrichment.
Minimizing the silver particle sizes is believed to be essential each from the stability of the colloidal suspension and for the efficacy against microbes.
Various processes to produce nanoparticles are recognized.

For example, these disclosed in U.S. Pat. Nos. 5,543,133 five,585,
020 5,879,750 and 6,540,495. CS Pro Systems advertises a high voltage AC processor creating
nanoparticles of colloidal metal.

X Colloidal Silver, Copper & Gold 500ml + No cost Pure Copper Bracelet

Purified (distilled) water

When Technologies Fails: Make Your Personal Footwear

2 Silver Rods .9999 pure, approx. six" length

Take ½ to 1 cup a day internally

[img]https://cdn3.volusion.com/pahfr.ubfkh/v/vspfiles/photos/SLGBK-3.jpg?1412018050[/img]Saw or Swiss army knife for cutting wood strips

The HVAC procedure is claimed to create particle sizes among .002 to .007-9 microns by imposing an AC prospective of ten,000 volts across two metal electrodes in a distilled water medium. Colloidal suspensions of nanoparticles of electrically conductive materials can also be made by generating a plasma reaction among two electrodes, comprising the preferred electrically conductive material(s), which are immersed inside a dense fluid medium. Preferably, the dense medium is swiftly recirculated amongst the two electrodes. For the present invention, the electrodes are silver. Typically, the electrically conducting material must be at least about 90 percent pure, preferably at least about 95 percent pure, extra preferably at least about 99 % pure.

Preferably, the electrodes are constructed so as to be very easily removed and installed. This straightforward interchangeability facilitates replacing worn electrodes or altering electrodes to accommodate the production of various colloidal dispersions. The dense medium might be any liquid possessing a viscosity low enough to permit fast circulation of the fluid involving the two electrodes. The plasma reaction will decompose the molecules of the dense medium into very reactive cost-free radicals. As such, the reaction goods formed from the dense medium totally free radicals may perhaps be undesirable contaminants to the colloidal solution. Preferably, the dense medium will not type undesirable by-solutions through the plasma reaction. An undesirable by-solution is any compound that must be removed, due to technical, practical or aesthetic reasons, from the colloidal dispersion prior to use. In most cases, when inorganic/organic hybrid nanoparticle systems are prepared, liquid phase organometallic compounds can be employed.

The dense medium is typically chosen to avoid the production of undesirable byproducts. OH−) readily react with every single other to reform the water molecule. In contrast, the decomposition reaction items of other dense media, e.g. benzene, are absolutely free radicals which may possibly initiate polymerization reactions. The dense medium, and any by-product of the plasma reaction, ought to have, at most, a slow reaction price with the electrically conductive material. Preferably, any reaction between the dense medium and the electrically conductive material is slow enough that the nanoparticles in the colloidal dispersion have the preferred shelf-life. Most preferably, the dense medium, and any by-solution, is non-reactive with the electrically conducting material.