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The net result of the in vitro studies indicated that pERK responded to gefitinib in a distinguishing and dose-dependent manner in the two glioma cell lines and thus might serve the same function in vivo.

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Another critical issue in method selection is the spectrum of mutations tested.

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NCI-H1975 cells were treated with gefitinib (3 µM) or V1801 (3 µM) for indicated time points, lysed, and Western blot analysis was performed.

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Epidemiology of lung cancer: ACCP evidence-based clinical practice guidelines (2nd edition).

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The same membranes were stripped and reblotted using an anti-pEGFR (Tyr 1045) and an anti-myc antibody to examine the phosphorylation and protein levels of the immunoprecipitated EGFRs, respectively.

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No dosage adjustment is required on the basis of patient age, body weight, gender, ethnicity, or renal function; or in patients with moderate to severe hepatic impairment due to liver metastases (see section).

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